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Antibodies

Mouse anti Myc-Tag monoclonal antibody

Product Sizes
50 ul
£145.00
AE010-50UL
100 ul
£205.00
AE010-100UL
200 ul
£318.00
AE010-200UL
500 ul
£639.00
AE010-500UL
1000 ul
£1151.00
AE010-1000UL
About this Product
SKU:
AE010
Additional Names:
Myc|Myc tag|Myc-tag
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG1
Purification:
Affinity Purified
Reactivities:
Label/Epitope-tag, Wide/Non-species Specific
Sequence:
EQKLISEEDL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Tagged Antibodies
  • Manufacturer's Data Sheet:AE010
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with NLK-Myc (C-terminal) or PTEN-Myc (C-terminal) using Mouse anti Myc-Tag mAb (AE010) at 1:10000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) .Exposure time: 30s.
Confocal imaging of 293F cells transfected with SQSTM1-Myc(N) cells using Mouse anti Myc-Tag mAb (AE010, dilution 1:2000) followed by a further incubation with Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.