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Antibodies

Rabbit anti Myc-Tag polyclonal antibody

Product Sizes
50 ul
£127.00
AE009-50UL
100 ul
£181.00
AE009-100UL
200 ul
£270.00
AE009-200UL
500 ul
£532.00
AE009-500UL
1000 ul
£948.00
AE009-1000UL
About this Product
SKU:
AE009
Additional Names:
Myc|Myc tag|Myc-tag
Application:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Label/Epitope-tag, Wide/Non-species Specific
Sequence:
EQKLISEEDL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Tagged Antibodies
  • Manufacturer's Data Sheet:AE009
Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein,using Rabbit anti Myc-Tag pAb (AE009) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunofluorescence analysis of HeLa-Myc-C and HeLa cells using Rabbit anti Myc-Tag pAb (AE009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 ug extracts of 293T cells using 3 ug Rabbit anti Myc-Tag pAb antibody (AE009). Western blot was performed from the immunoprecipitate using Rabbit anti Myc-Tag pAb antibody (AE009) at a dilution of 1:50000.
Immunoprecipitation analysis of 300 ug extracts of 293T-NLK-Myc cells using 3 ug Myc-Tag antibody (AE009). Western blot was performed from the immunoprecipitate using Myc-Tag antibody (AE009) at a dilution of 1:20000.