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Antibodies

Rabbit anti DDDDK-Tag polyclonal antibody

Product Sizes
50 ul
£127.00
AE004-50UL
100 ul
£181.00
AE004-100UL
200 ul
£270.00
AE004-200UL
500 ul
£532.00
AE004-500UL
1000 ul
£948.00
AE004-1000UL
About this Product
SKU:
AE004
Additional Names:
DDDDK|DDDDK tag|DDDDK-tag
Application:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Label/Epitope-tag, Wide/Non-species Specific
Sequence:
DYKDDDDK
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Tagged Antibodies
  • Manufacturer's Data Sheet:AE004
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with DDDDK-Tag using Rabbit anti DDDDK-Tag pAb (AE004) at 1:10000 dilution. Secondary antibody:(AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunofluorescence analysis of 293T cells transfected with Flag-c(mCherry) and 293T cells transfected with Flag-N(mCherry) use Rabbit anti DDDDK-Tag pAb (AE004) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of 293T cells transfected with Flag-N(mCherry) and untreated 293T cells use Rabbit anti DDDDK-Tag pAb (AE004) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 ug extracts of 293T-SERPINB1-Flag-GFP cells using 3 ug DDDDK-Tag pAb (AE004). Western blot was performed from the immunoprecipitate using DDDDK-Tag pAb (AE004) at a dilition of 1:50000.