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Antibodies

GAPDH Mouse monoclonal antibody

Product Sizes
50 ul
£145.00
AC002-50UL
100 ul
£205.00
AC002-100UL
200 ul
£258.00
AC002-200UL
500 ul
£532.00
AC002-500UL
1000 ul
£948.00
AC002-1000UL
About this Product
SKU:
AC002
Additional Names:
G3PD|GAPD|GAPDH|HEL-S-162eP
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG2b, kappa
Molecular Weight:
36kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:AC002
Western blot analysis of various lysates using GAPDH Mouse mAb (AC002) at 1:10000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Western blot analysis of lysates from HeLa cells using GAPDH Mouse mAb (AC002) at 1:5000-1:1280000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) Exposure time: 20 s.
Immunohistochemistry analysis of paraffin-embedded Human kidney using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.