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Antibodies

EAAT1/SLC1A3 Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
A9712-20UL
100 ul
£342.00
A9712-100UL
About this Product
SKU:
A9712
Additional Names:
EA6|EAAT1|EAAT1/SLC1A3|GLAST|GLAST1
Application:
ELISA, IHC-Paraffin, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
60kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
GTKENMHREGKIVRVTAADAFLDLIRNMFPPNLVEACFKQFKTNYEKRSFKVPIQANETLVGAVINNVSEAMETLTRITEELVPVPGSVNGVNALGLVVFS
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A9712
Western blot analysis of various lysates using EAAT1/SLC1A3 Rabbit mAb (A9712) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using EAAT1/SLC1A3 Rabbit mAb (A9712) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using EAAT1/SLC1A3 Rabbit mAb (A9712) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human brain tissue using EAAT1/SLC1A3 Rabbit mAb (A9712) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human brain tissue (left, Positive control) and Human colon tissue (right, Negative control) tissue using EAAT1/SLC1A3 Rabbit mAb (A9712) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.