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Antibodies

CD172a/SIRPA Alpha Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
A9545-20UL
100 ul
£342.00
A9545-100UL
About this Product
SKU:
A9545
Additional Names:
BIT|CD172A|CD172a/SIRPA Alpha|MFR|MYD-1|P84|PTPNS1|SHPS1|SIRP
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
55kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
RIRQKKAQGSTSSTRLHEPEKNAREITQDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSE
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A9545
Western blot analysis of various lysates using CD172a/SIRPA Alpha Rabbit mAb (A9545) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Western blot analysis of various lysates using CD172a/SIRPA Alpha Rabbit mAb (A9545) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunofluorescence analysis of U-2 OS cells using CD172a/SIRPA Alpha Rabbit mAb (A9545) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.