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Antibodies

CD82 Rabbit monoclonal antibody

Product Sizes
20 ul
£163.00
A9264-20UL
100 ul
£342.00
A9264-100UL
About this Product
SKU:
A9264
Additional Names:
4F9|C33|CD82|GR15|IA4|KAI1|R2|SAR2|ST6|TSPAN27
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
30kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Sequence:
PEVTYPCSCEVKGEEDNSLSVRKGFCEAPGNRTQSGNHPEDWPVYQEGCMEKVQAWLQENLGIILGVGVGVAIIELLGMVLSICLCRHVHSEDYSKVPKY
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A9264
Western blot analysis of various lysates, using CD82 Rabbit mAb (A9264) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD82 Rabbit mAb (A9264) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of RAW 264.7 cells using CD82 Rabbit mAb (A9264, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of Raji cells using CD82 Rabbit mAb (A9264, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.