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Antibodies

[KO Validated] KDM5B Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A7772-20UL
100 ul
£342.00
A7772-100UL
About this Product
SKU:
A7772
Additional Names:
5B|CT31|JARID1B|MRT65|PLU-1|PLU1|PPP1R98|PUT1|RBBP2H1A|RBP2-H1
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
176kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Sequence:
EESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEE
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A7772
Western blot analysis of lysates from wild type (WT) and KDM5B knockout (KO) 293T cells, using [KO Validated] KDM5B Rabbit pAb (A7772) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunofluorescence analysis of L929 cells using [KO Validated] KDM5B Rabbit pAb (A7772) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.