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Antibodies

Acetyl-Histone H3-K9 Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A7255-20UL
100 ul
£342.00
A7255-100UL
About this Product
SKU:
A7255
Additional Names:
Acetyl-Histone H3-K9|H3.4|H3/g|H3C16|H3FT|H3t|HIST3H3
Application:
ChIP, ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
15 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Other Species, Rat
Sequence:
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A7255
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at 1:1000 dilution. HeLa cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at 1:1000 dilution. NIH/3T3 cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from C6 cells, using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at 1:1000 dilution. C6 cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.