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Antibodies

POLD3 Rabbit polyclonal antibody

Product Sizes
20 ul
£152.00
A7243-20UL
100 ul
£336.00
A7243-100UL
500 ul
£1258.00
A7243-500UL
1000 ul
£2228.00
A7243-1000UL
About this Product
SKU:
A7243
Additional Names:
P66|P68|POLD3|PPP1R128
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
51kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Sequence:
ASKAAAKTQETNKETKTEAKEVTNASAAGNKAPGKGNMMSNFFGKAAMNKFKVNLDSEQAVKEEKIVEQPTVSVTEPKLATPAGLKKSSKKAEPVKVLQKEKKRGKRVALSDDETKETENMRKKRRRIKLPESDSSEDEVFPDSPGAYEAESPSPPPPPSPPLEPVPKTEPEPPSVKSSSGENKRKRKRVLKSKTYLDGEGCIVTEKVYESESCTDSEEELNMKTSSVHRPPAMTVKKEPREERKGPKKGTAALGKANRQVSITGFFQRK
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A7243
Western blot analysis of various lysates using POLD3 Rabbit pAb (A7243) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using POLD3 Rabbit pAb (A7243) at dilution of 1:300 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using POLD3 Rabbit pAb (A7243). Green : GFP-RNF168 fusion protein expression for DNA damage marker.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunofluorescence analysis of U2OS cells using POLD3 Rabbit pAb (A7243).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.