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Antibodies

[KO Validated] FUS Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A5921-20UL
100 ul
£342.00
A5921-100UL
About this Product
SKU:
A5921
Additional Names:
ALS6|altFUS|ETM4|FUS1|HNRNPP2|POMP75|TLS|US
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
53kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
TIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFSGNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGGRGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGGPGGSHMGGNYGDDRRGGRGGYDRGGYRGRGGDRGGFRGGRGGGDRGGFGPGKMDSRGEHRQDRRERPY
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A5921
Western blot analysis of lysates from wild type (WT) and FUS knockout (KO) 293T cells, using [KO Validated] FUS Rabbit pAb (A5921) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunofluorescence analysis of C6 cells using [KO Validated] FUS Rabbit pAb (A5921) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] FUS Rabbit pAb (A5921) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 ug extracts of Jurkat cells using 3 ug FUS antibody (A5921). Western blot was performed from the immunoprecipitate using FUS antibody (A5921) at a dilution of 1:2000.