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Antibodies

LC3A/LC3B Rabbit polyclonal antibody

Product Sizes
20 ul
£152.00
A5618-20UL
100 ul
£336.00
A5618-100UL
500 ul
£1258.00
A5618-500UL
1000 ul
£2228.00
A5618-1000UL
About this Product
SKU:
A5618
Additional Names:
ATG8E|ATG8F|LC3|LC3A|LC3A/LC3B|LC3B|MAP1A/1BLC3|MAP1ALC3|MAP1BLC3|MAP1LC3A|MAP1LC3B|MAP1LC3B-a
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
14kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNM
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A5618
Western blot analysis of various lysates, using LC3A/LC3B Rabbit pAb (A5618) at 1:2000 dilution. 293T,NIH/3T3 and C6 cells were treated with Chloroquine (50 uM) at 37°C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using LC3A/LC3B Rabbit pAb (A5618) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using LC3A/LC3B Rabbit pAb (A5618) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using LC3A/LC3B Rabbit pAb (A5618) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain using LC3A/LC3B Rabbit pAb (A5618) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.