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Antibodies

[KO Validated] S100A11 Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A5486-20UL
100 ul
£342.00
A5486-100UL
About this Product
SKU:
A5486
Additional Names:
11|HEL-S-43|MLN70|S100C
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
12kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
MAKISSPTETERCIESLIAVFQKYAGKDGYNYTLSKTEFLSFMNTELAAFTKNQKDPGVLDRMMKKLDTNSDGQLDFSEFLNLIGGLAMACHDSFLKAVPSQKRT
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A5486
Western blot analysis of various lysates using [KO Validated] S100A11 Rabbit pAb (A5486) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from wild type (WT) and S100A11 knockout (KO) HeLa cells, using [KO Validated] S100A11 Rabbit pAb (A5486) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using [KO Validated] S100A11 Rabbit pAb (A5486). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of THP-1 cells using 3 ug S100A11 antibody (A5486). Western blot was performed from the immunoprecipitate using S100A11 antibody (A5486) at a dilution of 1:1000.