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Antibodies

TEAD1 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A5218-20UL
100 ul
£342.00
A5218-100UL
500 ul
£1532.00
A5218-500UL
1000 ul
£2704.00
A5218-1000UL
About this Product
SKU:
A5218
Additional Names:
AA|NTEF-1|REF1|TCF-13|TCF13|TEAD-1|TEAD1|TEF-1
Application:
Cell-based/Functional Assay, ChIP, ELISA, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
48kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MEPSSWSGSESPAENMERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARR
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A5218
Western blot analysis of various lysates using TEAD1 Rabbit mAb (A5218) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunoprecipitation analysis of 600 ug extracts of Mouse skeletal muscle cells using 3 ug TEAD1 antibody (A5218). Western blot was performed from the immunoprecipitate using TEAD1 antibody (A5218) at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using TEAD1 antibody (A5218) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ Hep G2 cells with 1 ug TEAD1 Rabbit mAb (A5218), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of TEAD1 in representative gene loci (SLC7A5), as shown in figure.