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Antibodies

RUVBL1/TIP49A/PONTIN Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A5180-20UL
100 ul
£342.00
A5180-100UL
About this Product
SKU:
A5180
Additional Names:
ECP-54|ECP54|INO80H|NMP 238|NMP238|PONTIN|Pontin52|RUVBL1/TIP49A/PONTIN|RVB1|TIH1|TIP49|TIP49A
Application:
ChIP, ELISA, IHC-Paraffin, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
50kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
IPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A5180
Western blot analysis of various lysates using RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using RUVBL1/TIP49A/PONTIN Rabbit mAb (A5180) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RUVBL1/TIP49A/PONTIN Rabbit mAb antibody (A5180) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.