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Antibodies

PI3 Kinase p85 alpha Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A4992-20UL
100 ul
£342.00
A4992-100UL
500 ul
£1532.00
A4992-500UL
1000 ul
£2704.00
A4992-1000UL
About this Product
SKU:
A4992
Additional Names:
AGM7|GRB1|IMD36|p85|p85-ALPHA|p85alpha|PI3 Kinase p85 alpha
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
84kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
LQYLLKHFFKLSQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEILISTEWNERQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A4992
Western blot analysis of various lysates, using PI3 Kinase p85 alpha Rabbit mAb (A4992) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using PI3 Kinase p85 alpha Rabbit mAb (A4992) at dilution of 1:60 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain using PI3 Kinase p85 alpha Rabbit mAb (A4992) at dilution of 1:60 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Mouse spleen tissue using PI3 Kinase p85 alpha Rabbit mAb (A4992, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of NIH/3T3 cells using PI3 Kinase p85 alpha Rabbit mAb (A4992, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.