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Antibodies

CD20 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A4893-20UL
100 ul
£342.00
A4893-100UL
500 ul
£1532.00
A4893-500UL
1000 ul
£2704.00
A4893-1000UL
About this Product
SKU:
A4893
Additional Names:
B1|Bp35|CD20|CVID5|FMC7|LEU-16|S7
Application:
ELISA, Flow Cytometry, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
33 kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
LLAATEKNSRKCLVKGKMIMNSLSLFAAISGMILSIMDILNIKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIF
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A4893
Western blot analysis of various lysates, using CD20 Rabbit mAb (A4893) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): HeLa Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD20 Rabbit mAb (A4893) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using CD20 Rabbit mAb (A4893) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Confocal imaging of Daudi and Jurkat(Negative control) cells using CD20 Rabbit mAb (A4893,dilution 1:1000)(Red). DAPI was used for nuclear staining (blue). Objective: 100x.
Flow cytometry: 1X10^6 Human PBMC were surface-stained with Rabbit IgG isotype control (AC042,2 ug/mL,left) or CD20 Rabbit mAb (A4893,2 ug/mL,right), followed by APC conjugated goat anti-rabbit pAb staining. Cells in the lymphocyte gate were used for analysis.