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Antibodies

ERK1/2 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A4782-20UL
100 ul
£342.00
A4782-100UL
500 ul
£1532.00
A4782-500UL
1000 ul
£2704.00
A4782-1000UL
About this Product
SKU:
A4782
Additional Names:
5594/5595|ERK|ERK-2|ERK1/2|ERK2|ERT1|MAPK2|p38|p40|p41|p41mapk|p42-MAPK|P42MAPK|PRKM1|PRKM2
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
42 kDa/44 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
HTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A4782
Western blot analysis of various lysates using ERK1/2 Rabbit mAb (A4782) at 1:4000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ERK1/2 Rabbit mAb (A4782) at a dilution of 1:9600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using ERK1/2 Rabbit mAb (A4782) at a dilution of 1:9600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using ERK1/2 Rabbit mAb (A4782) at a dilution of 1:9600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using ERK1/2 Rabbit mAb (A4782) at a dilution of 1:9600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.