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Antibodies

14-3-3 sigma Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A4377-20UL
100 ul
£342.00
A4377-100UL
About this Product
SKU:
A4377
Additional Names:
14-3-3 sigma|YWHAS
Application:
ELISA, IHC-Paraffin, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
28kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Sequence:
MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTVL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A4377
Western blot analysis of various lysates using 14-3-3 sigma Rabbit mAb (A4377) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human colon using 14-3-3 sigma Rabbit mAb (A4377) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.