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Antibodies

Ikaros Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A3565-20UL
100 ul
£342.00
A3565-100UL
500 ul
£1532.00
A3565-500UL
1000 ul
£2704.00
A3565-1000UL
About this Product
SKU:
A3565
Additional Names:
CVID13|Hs.54452|IK1|IKAROS|LyF-1|LYF1|PPP1R92|PRO0758|ZNFN1A1
Application:
Cell-based/Functional Assay, ChIP, ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
58kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Sequence:
AEDLCKIGSERSLVLDRLASNVAKRKSSMPQKFLGDKGLSDTPYDSSASYEKENEMMKSHVMDQAINNAINYLGAESLRPLVQTPPGGSEVVPVISPMYQL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A3565
Western blot analysis of various lysates using Ikaros Rabbit mAb (A3565) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Immunofluorescence analysis of Jurkat cells using Ikaros Rabbit mAb (A3565) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Ikaros antibody (A3565) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ K562 cells with 1 ug Ikaros Rabbit mAb (A3565), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of Ikaros in representative gene loci (MICA), as shown in figure.