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Antibodies

[KO Validated] TBK1/NAK Rabbit monoclonal antibody

Product Sizes
20 ul
£169.00
A3458-20UL
100 ul
£360.00
A3458-100UL
500 ul
£1532.00
A3458-500UL
1000 ul
£2704.00
A3458-1000UL
About this Product
SKU:
A3458
Additional Names:
FTDALS4|IIAE8|NAK|T2K|TBK1/NAK
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
84kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKLFAIEEETTTRHKVLIMEFCPCGSLYTVLEE
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A3458
Western blot analysis of various lysates using [KO Validated] TBK1/NAK Rabbit mAb (A3458) at 1:4000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90 s.
Western blot analysis of lysates from wild type (WT) and TBK1/NAK knockout (KO) 293T cells using [KO Validated] TBK1/NAK Rabbit mAb (A3458) at 1:7000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90 s.
Western blot analysis of lysates from Raji cells using [KO Validated] TBK1/NAK Rabbit mAb (A3458) at 1:7000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90 s.
Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] TBK1/NAK Rabbit mAb (A3458) at a dilution of 1:750 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human testis tissue using [KO Validated] TBK1/NAK Rabbit mAb (A3458) at a dilution of 1:750 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.