Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

ALOX5 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A2877-20UL
100 ul
£342.00
A2877-100UL
500 ul
£1532.00
A2877-500UL
1000 ul
£2704.00
A2877-1000UL
About this Product
SKU:
A2877
Additional Names:
5-LO|5-LOX|5LPG|ALOX5|LOG5
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
78kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
CYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQS
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A2877
Western blot analysis of lysates from Raji cells, using ALOX5 Rabbit mAb (A2877) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from Rat lung, using ALOX5 Rabbit mAb (A2877) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human appendix using ALOX5 Rabbit mAb (A2877) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using ALOX5 Rabbit mAb (A2877) at a dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of U-2 OS cells using ALOX5 Rabbit mAb (A2877,dilution 1:100)(Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.