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Antibodies

Granzyme B Rabbit polyclonal antibody

Product Sizes
20 ul
£152.00
A2557-20UL
100 ul
£336.00
A2557-100UL
500 ul
£1258.00
A2557-500UL
1000 ul
£2228.00
A2557-1000UL
About this Product
SKU:
A2557
Additional Names:
C11|CCPI|CGL-1|CGL1|CSP-B|CSPB|CTLA1|CTSGL1|Granzyme B|HLP|SECT
Application:
ELISA, IHC-Paraffin, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
28kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Sequence:
YNPKNFSNDIMLLQLERKAKRTRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFK
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A2557
Western blot analysis of various lysates, using Granzyme B Rabbit pAb (A2557) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Granzyme B Rabbit pAb (A2557) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded mouse spleen using Granzyme B Rabbit pAb (A2557) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.