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Antibodies

Human IgM Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A24831-20UL
100 ul
£342.00
A24831-100UL
About this Product
SKU:
A24831
Additional Names:
AGM1|Human IgM|MU|VH
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
49kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
GSASAPTLFPLVSCENSPSDTSSVAVGCLAQDFLPDSITFSWKYKNNSDISSTRGFPSVLRGGKYAATSQVLLPSKDVMQGTDEHVVCKVQHPNGNKEKNVPLPVIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTGKPTLYNVSLVMSDTAGTCY
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Secondary Antibodies
  • Manufacturer's Data Sheet:A24831
Western blot analysis of various lysates, using Human IgM Rabbit mAb (A24831) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control(NC):Jurkat Exposure time: 15s.
Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using Human IgM Rabbit mAb (A24831) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Human IgM Rabbit mAb (A24831) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human B-cell lymphoma using Human IgM Rabbit mAb (A24831) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 9.0) prior to IHC staining.
Confocal imaging of Daudi cells using Human IgM Rabbit mAb (A24831, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.