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Antibodies

[KD Validated] CD98/SLC3A2 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A24735-20UL
100 ul
£342.00
A24735-100UL
500 ul
£1532.00
A24735-500UL
1000 ul
£2704.00
A24735-1000UL
About this Product
SKU:
A24735
Additional Names:
4F2|4F2HC|4T2HC|CD98|CD98/SLC3A2|CD98HC|MDU1|NACAE
Application:
ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
57kDa/61kDa/67kDa/68kDa/71kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
RAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A24735
Western blot analysis of various lysates, using [KD Validated] CD98/SLC3A2 Rabbit mAb (A24735) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
Western blot analysis of lysates from wild type (WT) and CD98/SLC3A2 knockdown (KD) HeLa cells using [KD Validated] CD98/SLC3A2 Rabbit mAb (A24735) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Confocal imaging of HeLa cells using [KD Validated] CD98/SLC3A2 Rabbit mAb (A24735,dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.