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Antibodies

COUP-TFII/NR2F2 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A24647-20UL
100 ul
£342.00
A24647-100UL
About this Product
SKU:
A24647
Additional Names:
ARP-1|ARP1|CHTD4|COUP-TFII/NR2F2|COUPTF2|COUPTFB|COUPTFII|NF-E3|SRXX5|SVP40|TFCOUP2
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
46kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
VSTWRDPQDEVPGSQGSQASQAPPVPGPPPGAPHTPQTPGQGGPASTPAQTAAGGQGGPGGPGSDKQQQQQHIECVVCGDKSSGKHYGQFTCEGCK
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A24647
Western blot analysis of various lysates, using COUP-TFII/NR2F2 Rabbit mAb (A24647) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC):MOLT-4 Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using COUP-TFII/NR2F2 Rabbit mAb (A24647) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen using COUP-TFII/NR2F2 Rabbit mAb (A24647) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug COUP-TFII/NR2F2 antibody (A24647 1:100). Western blot was performed from the immunoprecipitate using COUP-TFII/NR2F2 antibody (A24647) at a dilition of 1:1000.