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Antibodies

KIR2DL2/KIR2DL3 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A24577-20UL
100 ul
£342.00
A24577-100UL
500 ul
£1532.00
A24577-500UL
1000 ul
£2704.00
A24577-1000UL
About this Product
SKU:
A24577
Additional Names:
CD158b|CD158B2|GL183|killer cell immunoglobulin-like receptor 2DL3|KIR-023GB|KIR-K7b|KIR-K7c|KIR2DL2/KIR2DL3|KIR2DL3|KIR2DS5|KIRCL23|NKAT|NKAT2|NKAT2A|NKAT2B|p58
Application:
ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
27kDa/37kDa/38kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
HEGVHRKPSLLAHPGPLVKSEETVILQCWSDVRFQHFLLHREGKFKDTLHLIGEHHDGVSKANFSIGPMMQDLAGTYRCYGSVTHSPYQLSAPSDPLDIVITGLYEKPSLSAQPGPTVLAGESVTLSCSSRSSYDMYHLSREGEAHERRFSAGPKVNGTFQADFPLGPATHGGTYRCFGSFRDSPYEWSNSSDPLLVSVTGNPSNSWPSPTEPSSETGNPRHLH
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A24577
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with KIR2DL2, using KIR2DL2/KIR2DL3 Rabbit mAb (A24577) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Confocal imaging of 293F-KIR2DL3 and 293F cells using KIR2DL2/KIR2DL3 Rabbit mAb (A24577,dilution 1:200)(Red). DAPI was used for nuclear staining (blue). Objective: 100x.
Confocal imaging of CHO-KIR2DL2 and CHO cells using KIR2DL2/KIR2DL3 Rabbit mAb (A24577,dilution 1:200)(Red). DAPI was used for nuclear staining (blue). Objective: 100x.
Flow cytometry: 1X10^6 293F cells (negative control,left) and 293F(Transfection,right) cells were surface-stained with KIR2DL2/KIR2DL3 Rabbit mAb(A24577,2ug/mL,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,2ug/mL,blue line),followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 293F(Transfection) cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,2 ug/mL,left) or KIR2DL2/KIR2DL3 Rabbit mAb(A24577,2 ug/mL,right).