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Antibodies

ESRA Alpha Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A24421-20UL
100 ul
£342.00
A24421-100UL
500 ul
£1532.00
A24421-500UL
1000 ul
£2704.00
A24421-1000UL
About this Product
SKU:
A24421
Additional Names:
ER|ESRA Alpha
Application:
ChIP, ELISA, Flow Cytometry, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
66kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
LTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A24421
Western blot analysis of various lysates, using ESRA Alpha Rabbit mAb (A24421) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): SK-BR-3 Exposure time: 3s.
Chromatin immunoprecipitation analysis of extracts of MCF7 cells treated by B Beta-estradiol (10 nM, 45 minutes),using ESRA Alpha Rabbit mAb(A24421) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR.Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.