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Antibodies

Asymmetric DiMethyl-Histone H3-R17 Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A2421-20UL
100 ul
£342.00
A2421-100UL
About this Product
SKU:
A2421
Additional Names:
Asymmetric DiMethyl-Histone H3-R17|H3/j|H3C1|H3C10|H3C11|H3C2|H3C3|H3C4|H3C6|H3C7|H3C8|H3FJ|HIST1H3J
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
15kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Other Species, Rat
Sequence:
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A2421
Western blot analysis of lysates from HeLa cells using Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R17 antibody (A2421).
Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.