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Antibodies

CD62E/E-Selectin Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A23836-20UL
100 ul
£342.00
A23836-100UL
About this Product
SKU:
A23836
Additional Names:
CD62E|CD62E/E-Selectin|E-selectin|ELAM|ELAM1|ESEL|LECAM2|SELE
Application:
ELISA, Flow Cytometry, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
66kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
WSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYWIGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKDVGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSGLKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMETMQCMSSGEW
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A23836
Western blot analysis of Recombinant Human SELE/E-selectin/CD62E Protein (RP00293), using CD62E/E-Selectin Rabbit mAb (A23836) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 5ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Flow cytometry: 1X10^6 HUVEC cells (negative control, Left) and HUVEC cells(treated with TNF-A Alpha,Right) were surface-stained with CD62E/E-Selectin Rabbit mAb(A23836,2ug/mL,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,2ug/mL,blue line),followed by Alexa Fluor®647 conjugated goat anti-rabbit pAb (1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 HUVEC cells (untreated,left) and HUVEC cells (treated with 10 ng/ml TNF-A Alpha for 6 hours,right) were surface-stained with CD62E/E-Selectin Rabbit mAb (A23836,2 ug/mL), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.