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Antibodies

pan 14-3-3 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A23829-20UL
100 ul
£342.00
A23829-100UL
About this Product
SKU:
A23829
Additional Names:
14-3-3|14-3-3-zeta|1C5|GW128|HEL-S-1|HEL-S-3|HEL-S-93|HEL4|HS1|KCIP-1|pan 14-3-3|POPCHAS|YWHAA|YWHAD
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
27kDa/28KDa/29KDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MEKTELIQKAKLAEQAERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTDTSDKKLQLIKDYREKVESELRSICTTVLEL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A23829
Western blot analysis of various lysates, using pan 14-3-3 Rabbit mAb (A23829) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunofluorescence analysis of H-4-11-E cells using pan 14-3-3 Rabbit mAb (A23829) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using pan 14-3-3 Rabbit mAb (A23829) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.