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Antibodies

Phospholamban Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A23750-20UL
100 ul
£342.00
A23750-100UL
About this Product
SKU:
A23750
Additional Names:
CMD1P|CMH18|phospholamban|PLB|PLN
Application:
ELISA, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
6 kDa
Purification:
Affinity Purified
Reactivities:
Mouse, Rat
Sequence:
MEKVQYLTRSAIRRASTIEMPQQARQKLQNLFINFCLILICLLLICIIVMLL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A23750
Western blot analysis of lysates from Mouse heart using Phospholamban Rabbit mAb (A23750) at 1:5000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10 s.
Western blot analysis of lysates from Rat heart using Phospholamban Rabbit mAb (A23750) at 1:5000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10 s.
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse heart using Phospholamban Rabbit mAb (A23750) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.