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Antibodies

CD239/BCAM Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A23695-20UL
100 ul
£342.00
A23695-100UL
About this Product
SKU:
A23695
Additional Names:
AU|CD239|CD239/BCAM|LU|MSK19
Application:
ELISA, Flow Cytometry, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
67kda
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
EVRLSVPPLVEVMRGKSVILDCTPTGTHDHYMLEWFLTDRSGARPRLASAEMQGSELQVTMHDTRGRSPPYQLDSQGRLVLAEAQVGDERDYVCVVRAGAAGTAEATARLNVFAKPEATEVSPNKGTLSVMEDSAQEIATCNSRNGNPAPKITWYRNGQRLEVPVEMNPEGYMTSRTVREASGLLSLTSTLYLRLRKDDRDASFHCAAHYSLPEGRHGRLDSPTFHLTLHYPTEHVQFWVGSPSTPAGWVREGDTVQLLCRGDGSPSPEYTLFRLQDEQEEVLNVNLEGNLTLEGVTRGQSGTYGCRVEDYDAADDVQLSKTLELRVAYLDPLELSEGKVLSLPLNSSAVVNCSVHGLPTPALRWTKDSTPLGDGPMLSLSSITFDSNGTYVCEASLPTVPVLSRTQNFTLLVQGSPELKTAEIEPKADGSWREGDEVTLICSARGHPDPKLSWSQLGGSPAEPIPGRQGWVSSSLTLKVTSALSRDGISCEASNPHGNKRHVFHFGTVSPQTSQA
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A23695
Western blot analysis of various lysates, using CD239/BCAM Rabbit mAb (A23695) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
Flow cytometry: 1X10^6 SH-SY5Y cells (Low Expression,left) and SK-BR-3 cells (right) were surface-stained with CD239/BCAM Rabbit mAb(A23695,2ug/mL,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,2ug/mL,blue line),followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb(1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 SK-BR-3 cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,2 ug/mL,left) or CD239/BCAM Rabbit mAb(A23695,2 ug/mL,right).