Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

[KO Validated] Smad3 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A23491-20UL
100 ul
£342.00
A23491-100UL
500 ul
£1532.00
A23491-500UL
1000 ul
£2704.00
A23491-1000UL
About this Product
SKU:
A23491
Additional Names:
d3|hMAD-3|hSMAD3|HSPC193|HsT17436|JV15-2|LDS1C|LDS3|mad3|MADH3
Application:
ELISA, IHC-Paraffin, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
48kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
EVCVNPYHYQRVETPVLPPVLVPRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A23491
Western blot analysis of lysates from wild type (WT) and Smad3 knockout (KO) 293T cells, using [KO Validated] Smad3 Rabbit mAb (A23491) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from A-431 cells, using [KO Validated] Smad3 Rabbit mAb (A23491) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] Smad3 Rabbit mAb (A23491) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunoprecipitation analysis of 300 ug extracts of A-431 cells using 3 ug Smad3 antibody (A23491). Western blot was performed from the immunoprecipitate using Smad3 antibody (A23491) at a dilution of 1:500.