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Antibodies

HSP70 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A23457-20UL
100 ul
£342.00
A23457-100UL
500 ul
£1532.00
A23457-500UL
1000 ul
£2704.00
A23457-1000UL
About this Product
SKU:
A23457
Additional Names:
HEL-S-103|HSP70|HSP70-1|HSP70-1A|HSP70-2|HSP70.1|HSP70.2|HSP70I|HSP72|HSPA1
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
70 kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Plant, Rat
Sequence:
KITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELE
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A23457
Western blot analysis of various lysates, using HSP70 Rabbit mAb (A23457) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).. Exposure time: 30s.
Western blot analysis of lysates from HeLa cells using HSP70 Rabbit mAb (A23457) at 1:10000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Western blot analysis of various lysates using HSP70 Rabbit mAb (A23457) at 1:4000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10 s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using HSP70 Rabbit mAb (A23457) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of HeLa cells using HSP70 Rabbit mAb (A4777, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.