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Antibodies

EGR1 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A23424-20UL
100 ul
£342.00
A23424-100UL
500 ul
£1532.00
A23424-500UL
1000 ul
£2704.00
A23424-1000UL
About this Product
SKU:
A23424
Additional Names:
AT225|EGR1|G0S30|KROX-24|NGFI-A|TIS8|ZIF-268|ZNF225
Application:
ELISA, IHC-Paraffin, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
58kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
ADTGEQPYEHLTAESFPDISLNNEKVLVETSYPSQTTRLPPITYTGRFSLEPAPNSGNTLWPEPLFSLVSGLVSMTNPPASSSSAPSPAASSASASQSPPLSCAVPSNDSSPIYSAAPTFPTPNTDIFPEPQSQAFPGSAGTALQYPPPAYPAAKGGFQVP
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A23424
Western blot analysis of lysates from 293T cells using EGR1 Rabbit mAb (A23424) at 1:5000 dilution incubated at room temperature for 1.5 hours. 293T cells were treated with 20% FBS at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Western blot analysis of lysates from C2C12 cells using EGR1 Rabbit mAb (A23424) at 1:5000 dilution incubated at room temperature for 1.5 hours. C2C12 cells were treated with EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.