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Antibodies

Glypican 3 (GPC3) Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A23410-20UL
100 ul
£342.00
A23410-100UL
About this Product
SKU:
A23410
Additional Names:
DGSX|Glypican 3 (GPC3)|GTR2-2|MXR7|OCI-5|SDYS|SGB|SGBS|SGBS1
Application:
ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
66kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
GRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVHSPLKLLTSMAISVVCFFFLVH
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A23410
Western blot analysis of lysates from Hep G2 cells using Glypican 3 (GPC3) Rabbit mAb (A23410) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10 s.
Confocal imaging of Hep G2 cells using Glypican 3 (GPC3) Rabbit mAb (A23410, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.