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Antibodies

[KO Validated] KRAS Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A23382-20UL
100 ul
£342.00
A23382-100UL
500 ul
£1532.00
A23382-500UL
1000 ul
£2704.00
A23382-1000UL
About this Product
SKU:
A23382
Additional Names:
'C-K-RAS|AS|C-K-RAS|c-Ki-ras|c-Ki-ras2|CFC2|K-Ras|K-Ras 2|K-RAS2A|K-RAS2B|K-RAS4A|K-RAS4B|KI-RAS|KRAS1|KRAS2|NS|NS3|OES|RALD|RASK2
Application:
ELISA, IHC-Paraffin, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
22kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQRVEDAFYTLVREIRQYRLKKISKEEKTPGCVKIKKCII
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A23382
Western blot analysis of various lysates, using KRAS Rabbit mAb (A23382) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 45s.
Western blot analysis of lysates from wild type (WT) and KRAS knockout (KO) 293T cells, using KRAS Rabbit mAb (A23382) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KO Validated] KRAS Rabbit mAb (A23382) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] KRAS Rabbit mAb (A23382) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.