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Antibodies

SMARCA2 / BRM Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A23291-20UL
100 ul
£342.00
A23291-100UL
About this Product
SKU:
A23291
Additional Names:
BAF190|BIS|BRM|hBRM|hSNF2a|NCBRS|SMARCA2 / BRM|SNF2|SNF2L2|SNF2LA|Sth1p|SWI2
Application:
ChIP, ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 201-300 of human SMARCA2 (NP_003061.3).
Isotype:
IgG
Molecular Weight:
181kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
TLQLAVQGKRTLPGLQQQQQQQQQQQQQQQQQQQQQQQPQQQPPQPQTQQQQQPALVNYNRPSGPGPELSGPSTPQKLPVPAPGGRPSPAPPAAAQPPAA
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A23291
Western blot analysis of various lysates using SMARCA2 / BRM Rabbit mAb (A23291) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10 s.
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using SMARCA2 / BRM Rabbit mAb (A23291) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using SMARCA2 / BRM Rabbit mAb (A23291) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 ug extracts of Hela cells using 3 ug SMARCA2 / BRM Rabbit mAb (A23291). Western blot was performed from the immunoprecipitate using SMARCA2 / BRM Rabbit mAb (A23291) at a dilition of 1:5000.
Chromatin immunoprecipitation was performed with 20 ug of cross-linked chromatin from MCF7 cells treated with B Beta-estradiol (10 nM, 45 min), using 5 ug of SMARCA2 / BRM Rabbit mAb(A23291) and Rabbit IgG isotype control(AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.