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Antibodies

Puromycin Rabbit monoclonal antibody

Product Sizes
20 ul
£169.00
A23031-20UL
100 ul
£360.00
A23031-100UL
500 ul
£1532.00
A23031-500UL
1000 ul
£2704.00
A23031-1000UL
About this Product
SKU:
A23031
Application:
ELISA, Flow Cytometry, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
CAS Number:
58-58-2
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Chemical compounds corresponding to Puromycin.
Isotype:
IgG
Purification:
Affinity Purified
Reactivities:
Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A23031
Western blot analysis of various lysates using Puromycin Rabbit mAb (A23031) at 1:8000 dilution incubated overnight at 4°C. 293F and Raw 264.7 cells were treated with puromycin (20 ug/mL) at 37°C for 4 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded HeLa and HeLa-puromycin cells using Puromycin Rabbit mAb (A23031) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded C6 and C6-puromycin cells using Puromycin Rabbit mAb (A23031) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded RAW264.7 and RAW264.7-puromycin cells using Puromycin Rabbit mAb (A23031) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of HeLa cells (treated with Puromycin) and HeLa cells (untreated) using Puromycin Rabbit mAb (A23031, dilution 1:2000) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.