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Antibodies

Acetyl-Histone H4-K12 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A22754-20UL
100 ul
£342.00
A22754-100UL
500 ul
£1532.00
A22754-500UL
1000 ul
£2704.00
A22754-1000UL
About this Product
SKU:
A22754
Additional Names:
Acetyl-Histone H4-K12|FO108|H4|H4-16|H4/n|H4C1|H4C11|H4C12|H4C13|H4C15|H4C16|H4C2|H4C3|H4C4|H4C5|H4C6|H4C8|H4C9|H4F2|H4FN|HIST2H4|HIST2H4A
Application:
ChIP, Dot Blot, ELISA, IHC-Paraffin, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
11kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Other Species, Rat
Sequence:
MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYG
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A22754
Western blot analysis of various lysates, using Acetyl-Histone H4-K12 Rabbit mAb (A22754) at 1:1000 dilution. HeLa cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Western blot analysis of various lysates, using Acetyl-Histone H4-K12 Rabbit mAb (A22754) at 1:1000 dilution. NIH/3T3 cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Western blot analysis of lysates from C6 cells, using Acetyl-Histone H4-K12 Rabbit mAb (A22754) at 1:1000 dilution. C6 cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H4-K12 antibody (A22754) at 1:1000 dilution.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Acetyl-Histone H4-K12 Rabbit mAb (A22754) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.