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Antibodies

Acetyl-Histone H3-K18 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A22566-20UL
100 ul
£342.00
A22566-100UL
500 ul
£1532.00
A22566-500UL
1000 ul
£2704.00
A22566-1000UL
About this Product
SKU:
A22566
Additional Names:
Acetyl-Histone H3-K18|H3/A|H3C10|H3C11|H3C12|H3C2|H3C3|H3C4|H3C6|H3C7|H3C8|H3FA|HIST1H3A
Application:
ChIP, Dot Blot, ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
16kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Other Species, Rat
Sequence:
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A22566
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K18 Rabbit mAb (A22566) at 1:10000 dilution. HeLa cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from C6 cells, using Acetyl-Histone H3-K18 Rabbit mAb (A22566) at 1:10000 dilution. C6 cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K18 Rabbit mAb (A22566) at 1:10000 dilution. NIH/3T3 cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K18 antibody (A22566) at 1:10000 dilution.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Acetyl-Histone H3-K18 Rabbit mAb (A22566) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.