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Antibodies

DNMT1 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A22455-20UL
100 ul
£342.00
A22455-100UL
500 ul
£1532.00
A22455-500UL
1000 ul
£2704.00
A22455-1000UL
About this Product
SKU:
A22455
Additional Names:
ADCADN|AIM|CXXC9|DNMT|DNMT1|HSN1E|m.HsaI|MCMT
Application:
ELISA, IHC-Paraffin, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
183kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYN
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A22455
Western blot analysis of various lysates using DNMT1 Rabbit mAb (A22455) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Western blot analysis of various lysates using DNMT1 Rabbit mAb (A22455) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using DNMT1 Rabbit mAb (A22455) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using DNMT1 Rabbit mAb (A22455) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using DNMT1 Rabbit mAb (A22455) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.