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Antibodies

KRAS+HRAS+NRAS Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A22438-20UL
100 ul
£342.00
A22438-100UL
About this Product
SKU:
A22438
Additional Names:
C-K-RAS|c-Ki-ras2|CFC2|K-RAS2A|K-RAS2B|K-RAS4A|K-RAS4B|KI-RAS|KRAS+HRAS+NRAS|KRAS1|KRAS2|NS|NS3|RALD|RASK2
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
21kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQI
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A22438
Western blot analysis of various lysates, using KRAS+HRAS+NRAS Rabbit mAb (A22438) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunofluorescence analysis of NIH/3T3 cells using KRAS+HRAS+NRAS Rabbit mAb (A22438) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using KRAS+HRAS+NRAS Rabbit mAb (A22438) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using KRAS+HRAS+NRAS Rabbit mAb (A22438) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600 ug extracts of Mouse brain using 3 ug KRAS+HRAS+NRAS antibody (A22438). Western blot was performed from the immunoprecipitate using KRAS+HRAS+NRAS antibody (A22438) at a dilution of 1:1000.