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Antibodies

CD225/IFITM1 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A22196-20UL
100 ul
£342.00
A22196-100UL
About this Product
SKU:
A22196
Additional Names:
9-27|CD225|CD225/IFITM1|DSPA2a|IFI17|LEU13
Application:
ELISA, IHC-Paraffin, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
14kDa
Purification:
Affinity Purified
Reactivities:
Human
Sequence:
MHKEEHEVAVLGPPPSTILPRSTVINIHSETSVPDHVVWSLFNTLFLNWCCLGFIAFAYSVKSRDRKMVGDVTGAQAYAS
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A22196
Western blot analysis of various lysates, using CD225/IFITM1 Rabbit mAb (A22196) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD225/IFITM1 Rabbit mAb (A22196) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using CD225/IFITM1 Rabbit mAb (A22196) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal imaging of human tonsil using CD225/IFITM1 Rabbit mAb (A22196,at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.