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Antibodies

MYH14 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A21933-20UL
100 ul
£342.00
A21933-100UL
About this Product
SKU:
A21933
Additional Names:
DFNA4|DFNA4A|FP17425|MHC16|MYH14|MYH17|myosin|NMHC II-C|NMHC-II-C|PNMHH
Application:
ELISA, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
228kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
AGKVDYKANEWLMKNMDPLNDNVAALLHQSTDRLTAEIWKDVEGIVGLEQVSSLGDGPPGGRPRRGMFRTVGQLYKESLSRLMATLSNTNPSFVRCIVPNH
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A21933
Western blot analysis of lysates from mouse lung, using MYH14 Rabbit mAb (A21933) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Western blot analysis of various lysates using MYH14 Rabbit mAb (A21933) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Confocal imaging of paraffin-embedded Human colon cancer using MYH14 Rabbit mAb (A21933,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.