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Antibodies

[KO Validated] SUMO1 Rabbit polyclonal antibody

Product Sizes
20 ul
£164.00
A21643-20UL
100 ul
£342.00
A21643-100UL
About this Product
SKU:
A21643
Additional Names:
DAP1|GMP1|O1|OFC10|PIC1|SENP2|SMT3|SMT3C|SMT3H3|UBL1
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
12kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHST
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C 50% glycerol. Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A21643
Western blot analysis of lysates from wild type(WT) and SUMO1 Rabbit pAb knockout (KO) 293T cells, using [KO Validated] SUMO1 Rabbit pAb (A21643) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] SUMO1 Rabbit pAb (A21643) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain using [KO Validated] SUMO1 Rabbit pAb (A21643) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using [KO Validated] SUMO1 Rabbit pAb (A21643) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of C6 cells using [KO Validated] SUMO1 Rabbit pAb (A21643) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.