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Antibodies

[KO Validated] MSH6 Rabbit polyclonal antibody

Product Sizes
20 ul
£152.00
A21445-20UL
100 ul
£336.00
A21445-100UL
500 ul
£1258.00
A21445-500UL
1000 ul
£2228.00
A21445-1000UL
About this Product
SKU:
A21445
Additional Names:
GTBP|GTMBP|HNPCC5|HSAP|LYNCH5|MMRCS3|MSH-6|MSH6|p160
Application:
ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Polyclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
153kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Non-human Primate, Rat
Sequence:
MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPGGDAAWSEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKM
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:A21445
Western blot analysis of various lysates using MSH6 Rabbit pAb (A21445) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Western blot analysis of various lysates using [KO Validated] MSH6 Rabbit pAb (A21445) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) Exposure time: 10 s.
Western blot analysis of lysates from wild type (WT) and MSH6 knockout (KO) 293T cells using [KO Validated] MSH6 Rabbit pAb (A21445) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) Exposure time: 10 s.
Immunohistochemistry analysis of paraffin-embedded Human colon cancer (loss of MSH6 expression) using MSH6 Rabbit pAb (A21445) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MSH6 Rabbit pAb (A21445) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.