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Antibodies

Acetyl-Histone H3-K9 Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A21107-20UL
100 ul
£342.00
A21107-100UL
500 ul
£1532.00
A21107-500UL
1000 ul
£2704.00
A21107-1000UL
About this Product
SKU:
A21107
Additional Names:
Acetyl-Histone H3-K9|H3/A|H3C10|H3C11|H3C12|H3C2|H3C3|H3C4|H3C6|H3C7|H3C8|H3FA|HIST1H3A
Application:
Cell-based/Functional Assay, ChIP, Dot Blot, ELISA, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
15 kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Other Species, Rat
Sequence:
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A21107
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K-562 cells with 1 ug of Acetyl-Histone H3-K9 Rabbit mAb (A21107), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of Acetyl-Histone H3-K9 around RPL30 gene.
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K-562 with 1 ug of Acetyl-Histone H3-K9 Rabbit mAb (A21107), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of AAcetyl-Histone H3-K9 Rabbit across chromosome 8 (upper panel) and the genomic region encompassing RPL30, a representative gene enriched in Acetyl-Histone H3-K9 Rabbit (lower panel).
Western blot analysis of various lysates using Acetyl-Histone H3-K9 Rabbit mAb (A21107) at 1:30000 dilution incubated at room temperature for 1.5 hours. HeLa, NIH/3T3 and C6 cells were treated with TSA (1 uM) at 37°C for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1 s.
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K9 antibody (A21107) at 1:1000 dilution.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Acetyl-Histone H3-K9 Rabbit mAb (A21107) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.