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Antibodies

RAB8A Rabbit monoclonal antibody

Product Sizes
20 ul
£164.00
A20976-20UL
100 ul
£342.00
A20976-100UL
About this Product
SKU:
A20976
Additional Names:
MEL|RAB8|RAB8A
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
24kDa
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
RNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQGVKITPDQQKRSSFFRCVLL
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A20976
Western blot analysis of various lysates using RAB8A Rabbit mAb (A20976) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Western blot analysis of lysates from Rat spinal cord, using RAB8A Rabbit mAb (A20976) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Immunofluorescence analysis of NIH/3T3 cells using RAB8A Rabbit mAb (A20976) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using RAB8A Rabbit mAb (A20976) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600 ug extracts of Mouse brain cells using 3 ug RAB8A antibody (A20976). Western blot was performed from the immunoprecipitate using RAB8A antibody (A20976) at a dilution of 1:1000.